Use this Western Blot Densitometry Calculator to accurately quantify protein expression from your blot images. Input band and background intensities to normalize your target protein levels against a loading control, ensuring reliable and reproducible experimental data for your research.
Formula:
The calculator uses the following formula to determine Relative Band Intensity:
Relative Band Intensity = (Target Band Intensity - Target Background Intensity) / (Loading Control Band Intensity - Loading Control Background Intensity)
- Target Band Intensity: The pixel intensity value of your target protein band.
- Target Background Intensity: The pixel intensity value of the background area adjacent to your target protein band.
- Loading Control Band Intensity: The pixel intensity value of your loading control protein band.
- Loading Control Background Intensity: The pixel intensity value of the background area adjacent to your loading control protein band.