Unlock precise cellular analysis with our advanced Cell Count Calculator. Whether you're working with a hemocytometer, performing cell culture, or need accurate cell concentration and viability, this tool simplifies complex calculations. Get instant results for live cells per mL, total cells per mL, and viability percentage, crucial for microbiology and immunology research.
Formula:
Cell Concentration (cells/mL) Formula:
Concentration (cells/mL) = (Total Cells Counted / Number of Squares) × Dilution Factor × 104
Cell Viability (%) Formula:
Viability (%) = (Live Cells Counted / Total Cells Counted) × 100
- Live Cells Counted: The total number of viable cells observed across all counted squares.
- Dead Cells Counted: The total number of non-viable cells observed across all counted squares.
- Number of Squares: The total count of 1mm × 1mm squares used for counting (e.g., 4 or 9 large squares).
- Dilution Factor: The factor by which the original sample was diluted. Enter '1' if no dilution was performed (e.g., for a 1:2 dilution, enter '2').
- 104: A constant conversion factor used for hemocytometer calculations to convert cell counts to cells per milliliter (mL).