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Globulins are a group of proteins in your blood. They are made in your liver by your immune system. Globulins play an important role in liver function, blood clotting, and fighting infection. There are four main types of globulins. They are called alpha 1, alpha 2, beta, and gamma. Just as there are different types of globulins, there are different types of globulin tests. These include:
Total protein test: This blood test measures two types of proteins: globulin and albumin. If protein levels are low, it can mean that you have liver or kidney disease.
Serum protein electrophoresis: This blood test measures gamma globulins and other proteins in your blood. It can be used to diagnose a variety of conditions, including disorders of the immune system and a type of cancer called multiple myeloma.

This calculator calculates the globulin ratio using albumin, total protein values.