This calculator calculates the retardation factor using quantity of substance in the mobile phase, total quantity of substance in the system values.
In chromatography, the retardation factor (R) is the fraction of an analyte in the mobile phase of a chromatographic system.
Formula:
R = Qmb ÷ TQs
Where,Qmb = Quantity of substance in the mobile phase
TQs = Total quantity of substance in the system
R = Retardation factor