Accurately determine the volume of buffer required to resuspend your synthetic oligonucleotide (DNA/RNA) to a specific stock concentration. This essential tool simplifies lab calculations, ensuring precise results for molecular biology experiments like PCR and sequencing. Easily convert nmol to µM for your custom oligo.
Formula:
Volume (µL) = (Oligo Quantity (nmol) × 1000) ÷ Desired Concentration (µM)
Where:
Volume (µL) = The final volume of buffer (in microliters) required to achieve the desired concentration.
Oligo Quantity (nmol) = The total quantity of oligonucleotide, typically supplied by the manufacturer (in nanomoles).
Desired Concentration (µM) = The target stock concentration for your resuspension (in micromolar).