Easily calculate the exact resuspension volume in microliters (µL) for your DNA or RNA oligonucleotides. Our free Oligo Resuspension Calculator helps you achieve the desired molar concentration efficiently, saving time and ensuring accuracy in your lab work for applications like PCR, qPCR, and DNA sequencing.
Formula:
To determine the correct resuspension volume for your oligonucleotides, use the following formula:
Volume (µL) = (Amount of Oligo (nmol) * 1000) / Desired Concentration (µM)
Where:
- Amount of Oligo: The total quantity of your synthesized oligonucleotide, typically provided by the manufacturer in nanomoles (nmol).
- Desired Concentration: The target molarity (concentration) for your final oligo solution, commonly expressed in micromolar (µM).
- Volume: The calculated resuspension volume in microliters (µL) of buffer (e.g., TE buffer or nuclease-free water) required to achieve the desired concentration.