Michaelis-Menten Equation Calculator: Determine Enzyme Reaction Velocity

Calculate Initial Reaction Velocity (V)

Enter the maximum velocity of the reaction.
Enter the Michaelis constant, representing substrate concentration at half Vmax.
Enter the current substrate concentration.

Our Michaelis-Menten Equation Calculator simplifies enzyme kinetics, allowing you to quickly determine the initial reaction velocity (V) based on Vmax, Km, and substrate concentration. Essential for biochemistry students and researchers.

Formula:

The Michaelis-Menten equation describes the rate of enzymatic reactions as a function of substrate concentration. The formula is:

V = (Vmax × [S]) / (Km + [S])

Where:
  • V: Initial reaction velocity (e.g., µM/min)
  • Vmax: Maximum reaction velocity (e.g., µM/min)
  • [S]: Substrate concentration (e.g., µM)
  • Km: Michaelis constant (e.g., µM)

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