Use this online Kjeldahl method calculator to accurately estimate the soluble protein content in your food, feed, or other organic samples. Input your titration data, sample weight, and customize the nitrogen to protein conversion factor for precise results.
Formula:
The Kjeldahl method estimates protein content by first determining the nitrogen content and then converting it using a specific factor. The core steps are:
- Calculate Moles of Nitrogen (N):
Moles N = ((Volume of Titrant for Sample (mL) - Volume of Titrant for Blank (mL)) × Molarity of Titrant (mol/L)) / 1000
- Calculate Mass of Nitrogen (g):
Mass N (g) = Moles N × 14.007 (Atomic Weight of Nitrogen)
- Calculate Percentage Nitrogen (%N):
%N = (Mass N (g) / Weight of Sample (g)) × 100
- Calculate Percentage Protein (% Protein):
% Protein = %N × Nitrogen to Protein Conversion Factor